MCM7 Antikörper (AA 195-319)

Produktnummer ABIN6252451

Der Maus Monoklonal Anti-MCM7-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, MCM7 in Proben von Human zu detektieren.

Kurzübersicht für MCM7 Antikörper (AA 195-319) (ABIN6252451)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MCM7-1467

Produktdetails anti-MCM7 Antikörper

Bindungsspezifität: AA 195-319

Aufreinigung: Protein G affinity chromatography

Immunogen: A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the MCM7 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Andere Bezeichnung: MCM7

Hintergrund: MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Gen-ID: 4176

UniProt: P33993

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA