TGFA Antikörper (C-Term)

Produktnummer ABIN6252275

Dieses Anti-TGFA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TGFA in IHC, WB, IF und FACS. Geeignet für Human, Ratte, Maus und Rind (Kuh).

Kurzübersicht für TGFA Antikörper (C-Term) (ABIN6252275)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFA Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: MF9

Produktdetails anti-TGFA Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Protein G affinity chromatography

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF alpha

Hintergrund: TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Gen-ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway