Topoisomerase II alpha Antikörper

Produktnummer ABIN6251852

Dieses Anti-Topoisomerase II alpha-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Topoisomerase II alpha in WB, IHC, IF und FACS. Geeignet für Human.

Kurzübersicht für Topoisomerase II alpha Antikörper (ABIN6251852)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: TPM2A-2

Produktdetails anti-Topoisomerase II alpha Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human partial recombinant protein was used as the immunogen for this Topoisomerase II alpha antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: Topoisomerase II alpha

Hintergrund: This mAb recognizes a 170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases