PTEN Antikörper (N-Term)

Produktnummer ABIN6242545

Dieses Anti-PTEN-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PTEN in WB, IF und IHC (p). Geeignet für Human. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für PTEN Antikörper (N-Term) (ABIN6242545)

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTEN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB05082

Produktdetails anti-PTEN Antikörper

Bindungsspezifität: AA 69-104, N-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PTEN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-104 amino acids from the N-terminal region of human PTEN.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-PTEN Antikörper (ABIN6242545)

Agouni, Mody, Owen, Czopek, Zimmer, Bentires-Alj, Bence, Delibegović: "Liver-specific deletion of protein tyrosine phosphatase (PTP) 1B improves obesity- and pharmacologically induced endoplasmic reticulum stress." in: The Biochemical journal, Vol. 438, Issue 2, pp. 369-78, (2011) (PubMed).

Lehman, Waning, Batuello, Cipriano, Kadakia, Mayo: "Induction of apoptotic genes by a p73-phosphatase and tensin homolog (p73-PTEN) protein complex in response to genotoxic stress." in: The Journal of biological chemistry, Vol. 286, Issue 42, pp. 36631-40, (2011) (PubMed).

Details zu PTEN

Target: PTEN (Phosphatase and Tensin Homolog (PTEN))

Andere Bezeichnung: PTEN

Hintergrund: PTEN, (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), is a tumor suppressor implicated in a large number of human tumors. The PTEN phosphatase incorporates the catalytic motif (HCXXGXXRS/T) that is a signature of the protein tyrosine phosphatase family. Recombinant human PTEN is a dual phosphatase with ability to dephosphorylate both tyrosine and serine/threonine residues. PTEN functions primarily as a lipid phosphatase to regulate signal transduction pathways, with a primary target identified as phosphatidylinositol 3,4,5 trisphosphate. In addition, PTEN presents weak tyrosine phosphatase activity, which may downregulate signaling pathways involving focal adhesion kinase or Shc. PTEN negatively regulates activation of the serine/threonine kinase Akt/PKB by blocking its phosphorylation, thereby inhibiting the PI 3 kinase Akt signaling pathway, which is important for cell survival. In vivo, the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity. Mutations in PTEN are associated with several common cancers including prostate, brain and breast cancer, and with Cowden's disease, an autosomal dominant disorder conferring susceptibility to benign and malignant tumors. Germline mutations of PTEN are also linked Lhermitte-Duclos disease and Bannayan-Zonana syndrome. Mutations of PTEN occur in 60 to 80 % of prostate cancers. PTEN is also essential for embryonic development.

Molekulargewicht: 47166

NCBI Accession: NP_000305

UniProt: P60484

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagie, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling