ACTH Antikörper (AA 25-39)

Produktnummer ABIN6191091

Produktdetails anti-ACTH Antikörper

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Bindungsspezifität: AA 25-39

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTH Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Coating (Coat)

Verwendungszweck: Mouse Monoclonal anti-ACTH (CLIP/1418), Purified, with BSA

Produktmerkmale: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH), it does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Synthetic peptide corresponding to aa25-39 of human ACTH (NGAEDESAEAFPLEF)

Klon: CLIP-1418

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunofluorescence 1-2 μg/mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Kommentare:

Normal pituitary gland or pituitary tumor

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Andere Bezeichnung: ACTH (ACTH Produkte)

Substanzklasse: Hormone

Hintergrund: Adrenocorticotropin, alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta-Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC

Molekulargewicht: ACTH is ~5 kDa

Gen-ID: 5443, 1897

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity