Prostate Specific Antigen Antikörper (CF®405S)
Kurzübersicht für Prostate Specific Antigen Antikörper (CF®405S) (ABIN6190372)
Target
Alle Prostate Specific Antigen (PSA) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Mouse Monoclonal anti-PSA (Prostate Specific Antigen) (A67-B/E3), CF405S Conjugate
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Produktmerkmale
- This antibody recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb 1A7G6B6 for PSA tests. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Immunogen
- PSA (p30) from human sperm plasma
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Isotyp
- IgG1 kappa
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Applikationshinweise
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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Kommentare
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PC12 cells or normal prostate or prostate carcinoma
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 100 μg/mL
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Buffer
- PBS/0.1 % BSA/0.05 % azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Protect from light
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- Prostate Specific Antigen (PSA)
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Andere Bezeichnung
- PSA (Prostate Specific Antigen)
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Hintergrund
- Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin
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Molekulargewicht
- 33-34 kDa
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Gen-ID
- 354, 171995
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UniProt
- P07288
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Pathways
- Komplementsystem
Target
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