Neurofilament Antikörper (Biotin)

Produktnummer ABIN6188767

Der Maus Monoklonal Anti-Neurofilament-Antikörper wurde für FACS, IHC (f) und IF validiert. Er ist geeignet, Neurofilament in Proben von Human, Ratte, Huhn, Schwein und Maus zu detektieren.

Kurzübersicht für Neurofilament Antikörper (Biotin) (ABIN6188767)

Target: Neurofilament

Reaktivität: Human, Ratte, Huhn, Schwein, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Neurofilament Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Klon: NF421 NFL-736

Produktdetails anti-Neurofilament Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Neurofilament (H+L) (NF421 + NFL736), Biotin Conjugate

Produktmerkmale: This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1:25-1:50
• Optimal dilution for a specific application should be determined by user

Kommentare:

Brain, Neuroblastoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Neurofilament

Target: Neurofilament

Molekulargewicht: 200 kDa & 68 kDa

Gen-ID: 4744, 198760

UniProt: P12036