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Neurofilament Antikörper (CF®568)

Reaktivität: Human, Ratte, Maus, Huhn, Schwein FACS, IHC (f), IF Wirt: Maus Monoclonal NF421 NFL-736 CF®568
Produktnummer ABIN6188752
  • Target Alle Neurofilament Produkte
    Neurofilament
    Reaktivität
    • 26
    • 23
    • 20
    • 14
    • 14
    • 3
    Human, Ratte, Maus, Huhn, Schwein
    Wirt
    • 21
    • 7
    • 1
    Maus
    Klonalität
    • 21
    • 8
    Monoklonal
    Konjugat
    • 16
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Neurofilament Antikörper ist konjugiert mit CF®568
    Applikation
    • 17
    • 13
    • 8
    • 7
    • 7
    • 5
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
    Verwendungszweck
    Mouse Monoclonal anti-Neurofilament (H+L) (NF421 + NFL736), CF568 Conjugate
    Produktmerkmale
    This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)
    Klon
    NF421 NFL-736
    Isotyp
    IgG
  • Applikationshinweise
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1:25-1:50
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    Brain, Neuroblastoma

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    Neurofilament
    Abstract
    Neurofilament Produkte
    Molekulargewicht
    200 kDa & 68 kDa
    Gen-ID
    4744, 198760
    UniProt
    P12036
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