SUMO1 Antikörper (CF®647)

Produktnummer ABIN6186846

Produktdetails anti-SUMO1 Antikörper (CF®647)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SUMO1 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-SUMO 1 (SM1/495), CF647 Conjugate

Produktmerkmale: This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SUMO1 protein

Klon: SM1-495

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Predicted to show broad species reactivity
• Optimal dilution for a specific application should be determined by user

Kommentare:

Breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Andere Bezeichnung: SUMO 1 (SUMO1 Produkte)

Synonyme: DAP1 antikoerper, GMP1 antikoerper, OFC10 antikoerper, PIC1 antikoerper, SENP2 antikoerper, SMT3 antikoerper, SMT3C antikoerper, SMT3H3 antikoerper, UBL1 antikoerper, hm:zeh0670 antikoerper, wu:fb74c02 antikoerper, wu:fd12c02 antikoerper, zgc:65934 antikoerper, zgc:85634 antikoerper, SUMO1 antikoerper, dap1 antikoerper, gmp1 antikoerper, ofc10 antikoerper, pic1 antikoerper, senp2 antikoerper, smt3 antikoerper, smt3c antikoerper, sumo-1 antikoerper, sumo1 antikoerper, ubl1 antikoerper, xsumo-1 antikoerper, xsumo1 antikoerper, ARABIDOPSIS THALIANA SMALL UBIQUITIN-LIKE MODIFIER 1 antikoerper, ATSUMO1 antikoerper, F10M23.180 antikoerper, F10M23_180 antikoerper, SMALL UBIQUITIN-LIKE MODIFIER 1 antikoerper, SUM1 antikoerper, SUMO 1 antikoerper, small ubiquitin-like modifier 1 antikoerper, pigSUMO1 antikoerper, SENTRIN antikoerper, SMTP3 antikoerper, SUMO-1 antikoerper, Smt3C antikoerper, Ubl1 antikoerper, small ubiquitin-like modifier 1 antikoerper, small ubiquitin-like modifier 1 S homeolog antikoerper, small ubiquitin-related protein 1 antikoerper, SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) antikoerper, SMT3 suppressor of mif two 3 homolog 1 (yeast) antikoerper, small ubiquitin-like modifier 1 L homeolog antikoerper, Sumo1 antikoerper, SUMO1 antikoerper, sumo1 antikoerper, sumo1.S antikoerper, sumo-1 antikoerper, sumo1.L antikoerper

Molekulargewicht: 11.5 kDa (Monomer), 90 kDa (Heteromer)

Gen-ID: 7341, 596171

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway