beta Catenin Antikörper (CF®488A)

Produktnummer ABIN6183402

Dieser Maus Monoklonal Antikörper detektiert spezifisch beta Catenin in WB, IHC, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Huhn, Rind (Kuh) und Hund.

Kurzübersicht für beta Catenin Antikörper (CF®488A) (ABIN6183402)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Catenin Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 15B8

Produktdetails anti-beta Catenin Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-Catenin, beta (15B8), CF488A Conjugate

Produktmerkmale: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant chicken ?-catenin

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa or MCF-7 cells. Breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Andere Bezeichnung: Catenin, beta

Hintergrund: Cadherin associated protein), beta 1 88  kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Molekulargewicht: 92 kDa

Gen-ID: 1499, 476018

UniProt: P35222

Pathways: Peptide Hormone Metabolism