Cadherin-16 Antikörper (CF®568)

Produktnummer ABIN6182900

Dieses Anti-Cadherin-16-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cadherin-16 in IF, FACS und IHC (f). Geeignet für Human, Maus, Hund und Kaninchen.

Kurzübersicht für Cadherin-16 Antikörper (CF®568) (ABIN6182900)

Target: Cadherin-16 (CDH16)

Reaktivität: Human, Maus, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cadherin-16 Antikörper ist konjugiert mit CF®568

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CDH16-1071

Produktdetails anti-Cadherin-16 Antikörper (CF®568)

Verwendungszweck: Mouse Monoclonal anti-Ksp-Cadherin / CDH16 (CDH16/1071), CF568 Conjugate

Produktmerkmale: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CDH16 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Normal kidney or renal cell carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Cadherin-16

Target: Cadherin-16 (CDH16)

Andere Bezeichnung: Ksp-Cadherin / CDH16

Hintergrund: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody

Molekulargewicht: 130 kDa

Gen-ID: 1014, 513660

UniProt: O75309