GFAP Antikörper (CF®594)

Produktnummer ABIN6180668

Der CF®594-konjugierte Maus Monoklonal anti-GFAP Antikörper (Klon GA-5) (ABIN6180668) detektiert spezifisch GFAP in WB, IF, FACS und IHC (f). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Rind (Kuh), Schwein, Huhn und Kaninchen.

Kurzübersicht für GFAP Antikörper (CF®594) (ABIN6180668)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein, Huhn, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: GA-5

Produktdetails anti-GFAP Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-GFAP (GA-5), CF594 Conjugate

Produktmerkmale: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: GFAP isolated from pig spinal cord

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Brain or Astrocytoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Molekulargewicht: ~50 kDa

Gen-ID: 2670, 514227

UniProt: P14136