Cytokeratin 18 Antikörper (CF®640R)

Produktnummer ABIN6179166

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 18 in IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Schwein, Cat, Rind (Kuh), Hamster, Schaf, Pferd, Frettchen, Ziege, Marmoset und Affe.

Kurzübersicht für Cytokeratin 18 Antikörper (CF®640R) (ABIN6179166)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Cat, Rind (Kuh), Hamster, Schaf, Pferd, Frettchen, Ziege, Marmoset, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist konjugiert mit CF®640R

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: C-04

Produktdetails anti-Cytokeratin 18 Antikörper (CF®640R)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin 18 (C-04), CF640R Conjugate

Produktmerkmale: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Does not react with chicken or Xenopus, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7, HeLa cells, Breast Cancer

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: Cytokeratin 18

Hintergrund: Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

Molekulargewicht: 45 kDa

Gen-ID: 3875, 406013

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose