Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Rekombinanter Keratin Basic Antikörper (CF®488A)

Der Kaninchen Monoklonal Anti--Antikörper wurde für WB, IF, FACS und IHC validiert. Er ist geeignet, in Proben von Human zu detektieren.
Produktnummer ABIN6178804

Kurzübersicht für Rekombinanter Keratin Basic Antikörper (CF®488A) (ABIN6178804)

Target

Alle Keratin Basic Antikörper anzeigen
Keratin Basic

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 19
  • 11
  • 8
  • 6
  • 6
  • 5
  • 4
  • 2
  • 2
  • 1
Human

Wirt

  • 12
  • 8
Kaninchen

Klonalität

  • 20
Monoklonal

Konjugat

  • 13
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
CF®488A

Applikation

  • 18
  • 17
  • 15
  • 12
  • 8
  • 3
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon

KRTH-1576R
  • Verwendungszweck

    Recombinant Rabbit Monoclonal anti-Cytokeratin, Basic (KRTH/1576R), CF488A Conjugate

    Produktmerkmale

    This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Recombinant full-length human KRT76 protein

    Isotyp

    IgG kappa
  • Applikationshinweise

    Immunohistology (formalin) 0.25-0.5 μg/mL
    • Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    Kommentare

    Epithelial cells, Skin or Adenocarcinomas

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Protect from light
  • Target

    Keratin Basic

    Andere Bezeichnung

    Cytokeratin, Basic

    Hintergrund

    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

    Molekulargewicht

    52-67 kDa

    Gen-ID

    51350, 654392

    UniProt

    Q01546
Sie sind hier:
Chat with us!