CD79a Antikörper (AA 202-216)

Produktnummer ABIN6177753

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD79a in FACS, IF und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Schwein und Affe.

Kurzübersicht für CD79a Antikörper (AA 202-216) (ABIN6177753)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD79a Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: JCB117 HM47-A9

Produktdetails anti-CD79a Antikörper

Bindungsspezifität: AA 202-216

Verwendungszweck: Mouse Monoclonal anti-CD79a (JCB117 + HM47/A9), Purified, with BSA

Produktmerkmale: A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein (JCB117 and HM47/A9).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Daudi or Ramos cells. Germinal center B-cells in a lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Andere Bezeichnung: CD79a

Molekulargewicht: 44 kDa

Gen-ID: 973, 631567

UniProt: P11912

Pathways: BCR Signaling