BRCA1 Antikörper (AA 445-620) (CF®640R)

Produktnummer ABIN6175480

Der Maus Monoklonal Anti-BRCA1-Antikörper wurde für IF, FACS, ISt und IHC (f) validiert. Er ist geeignet, BRCA1 in Proben von Human zu detektieren.

Kurzübersicht für BRCA1 Antikörper (AA 445-620) (CF®640R) (ABIN6175480)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BRCA1 Antikörper ist konjugiert mit CF®640R

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: BRCA1-1398

Produktdetails anti-BRCA1 Antikörper (CF®640R)

Bindungsspezifität: AA 445-620

Verwendungszweck: Mouse Monoclonal anti-BRCA1 (BRCA1/1398), CF640R Conjugate

Produktmerkmale: The BRCA1 gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1

Molekulargewicht: 220 kDa

Gen-ID: 672, 194143

UniProt: P38398

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus