TGFA Antikörper (AA 34-43) (CF®647)

Produktnummer ABIN6171418

Der Maus Monoklonal Anti-TGFA-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, TGFA in Proben von Human, Kaninchen und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für TGFA Antikörper (AA 34-43) (CF®647) (ABIN6171418)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFA Antikörper ist konjugiert mit CF®647

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: P-T1

Produktdetails anti-TGFA Antikörper (CF®647)

Bindungsspezifität: AA 34-43

Verwendungszweck: Mouse Monoclonal anti-TGF alpha (P/T1), CF647 Conjugate

Produktmerkmale: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A 10-amino acid synthetic peptide (aa34-43, PPVAAAVVSH) from human TGF?.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-paraffin 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat or Ramos cells. Testicular Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF alpha

Hintergrund: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Molekulargewicht: 6 kDa

Gen-ID: 7039, 170009

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway