CD31 Antikörper

Produktnummer ABIN6169356

Dieses Anti-CD31-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD31 in FACS, IF und IHC (f). Geeignet für Human, Kaninchen und Cynomolgus.

Kurzübersicht für CD31 Antikörper (ABIN6169356)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Human, Kaninchen, Cynomolgus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: JC70-A C31-3

Produktdetails anti-CD31 Antikörper

Verwendungszweck: Mouse Monoclonal anti-CD31 / PECAM-1 (JC70/A + C31.3), Purified, with BSA

Produktmerkmale: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Human recombinant CD31 protein (C31.3) & Membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat cells. Tonsil or Angiosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: CD31 / PECAM-1

Hintergrund: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',

Molekulargewicht: ~100 kDa (endothelium) and ~130 kDa (platelets)

Gen-ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization