VEGFA Antikörper (Biotin)

Produktnummer ABIN6168150

Dieses Maus Monoklonal-Antikörper erkennt spezifisch VEGFA in IF und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Hund und Kaninchen.

Kurzübersicht für VEGFA Antikörper (Biotin) (ABIN6168150)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VEGFA Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: VEGF-1063

Produktdetails anti-VEGFA Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-VEGF-A (VEGF/1063), Biotin Conjugate

Produktmerkmale: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human VEGF189 recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Tumor cells in astrocytomas, breast or ovarian carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGF-A

Hintergrund: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA

Molekulargewicht: 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)

Gen-ID: 7422, 73793

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling