HLA-A Antikörper (CF®647)

Produktnummer ABIN6164063

Dieses Anti-HLA-A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HLA-A in FACS und IF. Geeignet für Human.

Kurzübersicht für HLA-A Antikörper (CF®647) (ABIN6164063)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-A Antikörper ist konjugiert mit CF®647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 108-2C5

Produktdetails anti-HLA-A Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-HLA-A (MHC-I) (108-2C5), CF647 Conjugate

Produktmerkmale: HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the i±1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the i±1 domain was demonstrated in the formation of an intralocus HLA-A determinant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Normal human peripheral blood lymphocytes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

THP-1 and Jurkat cells. Tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Andere Bezeichnung: HLA-A (MHC-I)

Hintergrund: HLA-A, HLA class 1A, MHC class I HLA-A

Molekulargewicht: ~41 kDa

Gen-ID: 3105, 181244, 654404

UniProt: P01889, P30443, P30499

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response