FOXA1 Antikörper (AA 467-614) (CF®647)

Produktnummer ABIN6163303

Produktdetails anti-FOXA1 Antikörper (CF®647)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Bindungsspezifität: AA 467-614

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FOXA1 Antikörper ist konjugiert mit CF®647

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Verwendungszweck: Mouse Monoclonal anti-FOXA1 / HNF3A (FOXA1/1518), CF647 Conjugate

Produktmerkmale: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (? and ?), HNF-3 (?, ? and ?), HNF-4 (? and ?), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FOXA1 protein fragment (aa467-614) (exact sequence is proprietary)

Klon: FOXA1-1518

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Andere Bezeichnung: FOXA1 / HNF3A (FOXA1 Produkte)

Synonyme: HNF3A antikoerper, TCF3A antikoerper, Hnf-3a antikoerper, Hnf3a antikoerper, Tcf-3a antikoerper, Tcf3a antikoerper, fkd7 antikoerper, forkhead-7 antikoerper, HNF3-alpha antikoerper, HNF3alpha antikoerper, fkh2 antikoerper, foxa1 antikoerper, hnf3a antikoerper, tFoxA1 antikoerper, tcf3a antikoerper, xfkh2 antikoerper, fkh antikoerper, FOXA1 antikoerper, Ci-fkh antikoerper, HNF-3a antikoerper, forkhead box A1 antikoerper, forkhead box A1 S homeolog antikoerper, forkhead homolog antikoerper, forkhead box A1 L homeolog antikoerper, FOXA1 antikoerper, Foxa1 antikoerper, foxa1 antikoerper, foxa1.S antikoerper, fkh antikoerper, foxa1.L antikoerper

Hintergrund: Forkhead box protein A1 (FOXA1), Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A), Transcription factor 3A (TCF-3A or TCF3A)

Molekulargewicht: 79 kDa

Gen-ID: 3169, 163484

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis