KIT Ligand Antikörper (AA 26-189)

Produktnummer ABIN616022

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KIT Ligand in WB. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für KIT Ligand Antikörper (AA 26-189) (ABIN616022)

Target: KIT Ligand (KITLG)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-KIT Ligand Antikörper

Bindungsspezifität: AA 26-189

Spezifität: This antibody detects KITLG / SCF.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Protein-A purified

Immunogen: Highly pure (>95%) recombinant mouse SCF (Lys26-Ala189) derived from E. coli

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: KITLG / SCF

Hintergrund: Stem cell factor (SCF), also known as ckit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93 % aa sequence identity with rat SCF and 72 % 75 % with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/ckit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gen-ID: 17311

NCBI Accession: NP_038626

UniProt: P20826

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung