NGFR Antikörper (CF®488A)

Produktnummer ABIN6158377

Der Maus Monoklonal Anti-NGFR-Antikörper wurde für FACS, IF und IHC (f) validiert. Er ist geeignet, NGFR in Proben von Human, Kaninchen, Cat, Frettchen, Affe und Pavian zu detektieren.

Kurzübersicht für NGFR Antikörper (CF®488A) (ABIN6158377)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Human, Kaninchen, Cat, Frettchen, Affe, Pavian

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFR Antikörper ist konjugiert mit CF®488A

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: NGFR5

Produktdetails anti-NGFR Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-NGFR (Nerve-Growth Factor Receptor) (NGFR5), CF488A Conjugate

Produktmerkmale: This antibody recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: NGFR from A875 melanoma cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Does not react with mouse or rat, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: NGFR (Nerve-Growth Factor Receptor)

Molekulargewicht: 75 kDa

Gen-ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding