PLAP Antikörper (CF®405S)

Produktnummer ABIN6155923

Der Maus Monoklonal Anti-PLAP-Antikörper wurde für FACS, IF und IHC (fro) validiert. Er ist geeignet, PLAP in Proben von Human zu detektieren.

Kurzübersicht für PLAP Antikörper (CF®405S) (ABIN6155923)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist konjugiert mit CF®405S

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ALPP-238

Produktdetails anti-PLAP Antikörper (CF®405S)

Verwendungszweck: Mouse Monoclonal anti-PLAP (Placental Alkaline Phosphatase) (ALPP/238), CF405S Conjugate

Produktmerkmale: PLAP is a tissue specific, trophoblast-derived, 70 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2 metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This MAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human ALPP protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology frozen 1-2 μg/mL for 30 minutes at RT

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG2 cells. Placenta or seminoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: PLAP (Placental Alkaline Phosphatase)

Molekulargewicht: 70 kDa

Gen-ID: 250, 284255

UniProt: P05187