TOP1MT Antikörper (CF®640R)

Produktnummer ABIN6154255

Der Maus Monoklonal Anti-TOP1MT-Antikörper wurde für IF, FACS und IHC (f) validiert. Er ist geeignet, TOP1MT in Proben von Human zu detektieren.

Kurzübersicht für TOP1MT Antikörper (CF®640R) (ABIN6154255)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TOP1MT Antikörper ist konjugiert mit CF®640R

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: TOP1MT-488

Produktdetails anti-TOP1MT Antikörper (CF®640R)

Verwendungszweck: Mouse Monoclonal anti-Topoisomerase-I, MT (TOP1MT/488), CF640R Conjugate

Produktmerkmale: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline  pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human TOP1MT protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431 cells. Heart, Skeletal muscle, brain or fetal liver.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Andere Bezeichnung: Topoisomerase-I, MT

Hintergrund: DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase

Molekulargewicht: 70 kDa

Gen-ID: 116447, 528574

UniProt: Q969P6