CTNNB1 Antikörper (AA 36-44)

Produktnummer ABIN600589

Dieser Maus Monoklonal Antikörper detektiert spezifisch CTNNB1 in WB, IHC, ELISA und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für CTNNB1 Antikörper (AA 36-44) (ABIN600589)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: AA 36-44

Spezifität: Specific for the active form of beta-catenin, non-phosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species cross-reactivity: Mouse and rat (100 % ). Predicted to cross-react with frog and chicken (97/100), porcine (99/100) and zebrafish (91/100) based on epitope sequence homology. Does not cross-react with Drosophila. Others not tested.

Aufreinigung: Protein G purified

Immunogen: Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.

Type of Immunogen: Fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects beta-catenin in RIPA lysates from A431 cells. Immunohistochemistry: Detects b-catenin in mouse embryo sections. Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed with methanol.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide. See C2069-47

Konservierungsmittel: Sodium azide, Without preservative

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 1 year at -20°C.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1 / Beta Catenin

Hintergrund: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gen-ID: 1499

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2