CTNNB1 Antikörper (AA 36-44)

Produktnummer ABIN600589

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CTNNB1 in WB, IHC, ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für CTNNB1 Antikörper (AA 36-44) (ABIN600589)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: AA 36-44

Spezifität: Specific for the active form of beta-catenin, non-phosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species cross-reactivity: Mouse and rat (100 % ). Predicted to cross-react with frog and chicken (97/100), porcine (99/100) and zebrafish (91/100) based on epitope sequence homology. Does not cross-react with Drosophila. Others not tested.

Aufreinigung: Protein G purified

Immunogen: Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.

Type of Immunogen: Fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects beta-catenin in RIPA lysates from A431 cells. Immunohistochemistry: Detects b-catenin in mouse embryo sections. Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed with methanol.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide. See C2069-47

Konservierungsmittel: Sodium azide, Without preservative

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 1 year at -20°C.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1 / Beta Catenin

Hintergrund: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gen-ID: 1499

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2