SMAD3 Antikörper (Thr179)

Produktnummer ABIN5957461

Der Kaninchen Polyklonal anti-SMAD3 Antikörper (ABIN5957461) detektiert spezifisch SMAD3 in WB, ELISA, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für SMAD3 Antikörper (Thr179) (ABIN5957461)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: Thr179

Spezifität: DKFZP586N0721,DKFZp686J10186,hMAD 3,hMAD-3,hSMAD3,HSPC193,HST17436,JV15 2,JV15-2,JV152,LDS1C,LDS3,MAD (mothers against decapentaplegic Drosophila) homolog 3,MAD homolog 3,Mad homolog JV15 2,Mad protein homolog,MAD,mothers against decapentaplegic homolog 3,Mad3,MADH 3,MADH3,MGC60396,Mothers against decapentaplegic homolog 3,Mothers against DPP homolog 3,SMA and MAD related protein 3,SMAD 3,SMAD,SMAD family member 3,SMAD,mothers against DPP homolog 3,Smad3,SMAD3

Aufreinigung: Affinity purification

Immunogen: Synthesized peptide derived from human Smad3 around the non-phosphorylation site of Thr179.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:10000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: Smad3

Hintergrund: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Molekulargewicht: 48kDa

Gen-ID: 4088

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie