EGFR Antikörper (Tyr1197)

Produktnummer ABIN5956473

Der Kaninchen Polyklonal anti-EGFR Antikörper wird verwendet zum Nachweis von EGFR in Proben von Human, Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für EGFR Antikörper (Tyr1197) (ABIN5956473)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: Tyr1197

Spezifität: Avian erythroblastic leukemia viral (v erb b) oncogene homolog,Cell growth inhibiting protein 40,Cell proliferation inducing protein 61,EGF R,EGFR,EGFR,Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog),Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian),Epidermal growth factor receptor,erb-b2 receptor tyrosine kinase 1,ERBB,ERBB1,Errp,HER1,mENA,NISBD2,Oncogen ERBB,PIG61,Proto-oncogene c-ErbB-1,Receptor tyrosine protein kinase ErbB 1,Receptor tyrosine-protein kinase ErbB-1,SA7,Species antigen 7,Urogastrone,v-erb-b Avian erythroblastic leukemia viral oncogen homolog,wa2,Wa5

Aufreinigung: Affinity purification

Immunogen: Synthesized peptide derived from human EGFR around the non-phosphorylation site of Tyr1197.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, ELISA 1:20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol,  pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR

Hintergrund: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.

Molekulargewicht: 134kDa

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine