AIM2 Antikörper (C-Term)

Produktnummer ABIN571131

Dieses Anti-AIM2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von AIM2 in WB, ELISA, IHC, IF und FACS. Geeignet für Human.

Kurzübersicht für AIM2 Antikörper (C-Term) (ABIN571131)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AIM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-AIM2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: AIM2

Sequenz: GEKLQLTSGV HSTIK

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Andere Bezeichnung: AIM2

Hintergrund: AIM2, absent in melanoma 2, PYHIN4

Molekulargewicht: 39.0kDa

Gen-ID: 9447

NCBI Accession: NP_004824

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome