Retinoblastoma Protein (Rb) Antikörper

Produktnummer ABIN5708007

Der Kaninchen Monoklonal Anti-Retinoblastoma Protein (Rb)-Antikörper wurde für IHC (p) validiert. Er ist geeignet, Retinoblastoma Protein (Rb) in Proben von Human und Maus zu detektieren.

Kurzübersicht für Retinoblastoma Protein (Rb) Antikörper (ABIN5708007)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Retinoblastoma Protein (Rb) Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB1-2313R

Produktdetails anti-Retinoblastoma Protein (Rb) Antikörper

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Details zu Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Andere Bezeichnung: Retinoblastoma / Rb

Hintergrund: Recognizes a 105  kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Pathways: Mitotic G1-G1/S Phases