SIRT1 Antikörper (C-Term)

Produktnummer ABIN5706832

Hersteller Produkt- Nr.: 600-401-gw4

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SIRT1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SIRT1 Antikörper (C-Term) (ABIN5706832)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SIRT1 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-gw4

Hersteller: Rockland

Verwendungszweck: SIRT1 Antibody

Kreuzreaktivität (Details): Anti-SIRT1 is directed against human SIRT1.

Produktmerkmale: Synonyms: rabbit anti-SIRT1 antibody, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SirtT1 75 kDa fragment, 75SirT1, SIRT-1, SIRT 1, SIR2L1

Aufreinigung: This product is an affinity purified antibody produced by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-SIRT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal of human SIRT1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~62 or 81.7 kDa in size corresponding to SIRT1 by western blotting in the appropriate cell lysate or extract. Higher observed band ~125 kda is due to complexing proteins.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 5.0 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.00 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: SIRT1

Hintergrund: Background: SIRT1 antibody detects human SIRT1 protein. SIRT1 is a member of the sirtuin family of protein-modifying enzymes. SIRT1 is a NAD+-dependent deacetylase that plays an important role in many cellular processes. SIRT1 protein is known to regulate epigenetic gene silencing and suppress recombination of rDNA. SIRT1 deacetylates a wide range of substrates, including p53, NF-kB, FOXO transcription factors, and PGC-1 alpha, with roles in cellular processes such as muscle differentiation, adipogenesis, protection from axonal degeneration, and life span extension. SIRT1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, SIRT1 de-acetylates and affects the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors. Anti-SIRT1 Antibody is significant for researchers involved in research areas including cancer, diabetes, aging, neurodegenerative, metabolic and cardiovascular diseases.

Gen-ID: 23411

NCBI Accession: NP_001135970

UniProt: Q96EB6

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling