IL-10 Antikörper

Produktnummer ABIN5706825

Hersteller Produkt- Nr.: 109-401-312-0100

Dieses Anti-IL-10-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IL-10 in WB, IHC und ELISA. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für IL-10 Antikörper (ABIN5706825)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-IL-10 Antikörper

Hersteller Produkt- Nr.: 109-401-312-0100

Hersteller: Rockland

Verwendungszweck: IL-10 Antibody

Produktmerkmale: Synonyms: CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, rabbit anti-interleukin 10 antibody, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody, rabbit anti-IL-10

Aufreinigung: The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie.IL-1 stimulated IL-10 production from fibroblasts).

Sterilität: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with human IL-10.

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: IL-10 antiserum has been tested for use in immunoblotting. Although not tested, this antibody may be useful in radioimmunoassay, neutralizations, immunoprecipitation, ELISA and immunohistochemistry.  Reactivity in other immunoassays is unknown. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-IL-10 Antikörper (ABIN5706825)

Said, Dupuy, Trautmann, Zhang, Shi, El-Far, Hill, Noto, Ancuta, Peretz, Fonseca, Van Grevenynghe, Boulassel, Bruneau, Shoukry, Routy, Douek, Haddad, Sekaly: "Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection." in: Nature medicine, Vol. 16, Issue 4, pp. 452-9, (2010) (PubMed).

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: IL10

Hintergrund: Background: Anti-IL-10 Antibody recognizes IL-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), that is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. IL-10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract and indeed patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin 10, showing the importance of interleukin 10 for counteracting excessive immunity in the human body. Anti-IL-10 cytokine antibody is ideal for investigators involved Immunology and Signal Transduction research.

Gen-ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints