Retinoblastoma 1 Antikörper (meLys860)

Produktnummer ABIN5706809

Hersteller Produkt- Nr.: 600-401-gz4

Produktdetails anti-Retinoblastoma 1 Antikörper

Target: Retinoblastoma 1 (RB1)

Bindungsspezifität: meLys860

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB)

Hersteller Produkt- Nr.: 600-401-gz4

Hersteller: Rockland

Verwendungszweck: Retinoblastoma K860 Me1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Retinoblastoma.

Produktmerkmale: Synonyms: Retinoblastoma-associated protein, p105-Rb, pRb, pp110, RB Transcriptional Co-repressor, Protein Phosphatase 1 Regulatory Subunit 130, Prepro-Retinoblastoma-Associated Protein, Retinoblastoma, P105-Rb, Retinoblastoma Susceptibility Protein, Retinoblastoma-Associated Protein, Osteosarcoma, PPP1R130, OSRC

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Retinoblastoma K860 Me1 Antibody was tested for use in ELISA, Dot Blot, and Western Blot. Expect a band at approximately 106.1 kDa in MOLT-4 and other appropriate cell lysates or tissues. Although not tested, expect reactivity in immunoprecipitation and immunohistochemistry.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 1:10,000-1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.99 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: RB1 (RB1 Produkte)

Hintergrund: Background: RB1 (RB Transcriptional Corepressor 1) is a protein coding gene. The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The active, hypo-phosphorylated form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. RB1 is directly involved in heterochromatin formation by maintaining overall chromatin structure and the constitutive heterochromatin, by stabilizing histone methylation. Retinoblastoma recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 Lys-20 trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein, or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1s activity. RB1 may be associated with diseases such as retinoblastoma, small cell cancer of the lung, bladder cancer, and osteogenic sarcoma. Anti-Retinoblastoma K860 Me1 Antibody is useful for researchers interested in cancer, transcription factor, epigenetics, and enzyme antibody research.

Gen-ID: 5925

NCBI Accession: NP_000312

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie