ADO Antikörper (AA 49-261) (DyLight 488)

Produktnummer ABIN7354745

Dieses Anti-ADO-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ADO in FACS. Geeignet für Human.

Kurzübersicht für ADO Antikörper (AA 49-261) (DyLight 488) (ABIN7354745)

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADO Antikörper ist konjugiert mit DyLight 488

Applikation: Flow Cytometry (FACS)

Produktdetails anti-ADO Antikörper (DyLight 488)

Bindungsspezifität: AA 49-261

Verwendungszweck: Anti-Human ADO DyLight® 488 conjugated Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Human ADO DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Details zu ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Andere Bezeichnung: ADO

Hintergrund:

Synonyms: 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22

Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers.

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Molekulargewicht: 39 kDa