14-3-3 sigma/SFN Antikörper (AA 1-248)

Produktnummer ABIN5693156

Zitiert in 1 Publikation. Dieser Anti-14-3-3 sigma/SFN-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von 14-3-3 sigma/SFN in WB, IHC, ELISA, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für 14-3-3 sigma/SFN Antikörper (AA 1-248) (ABIN5693156)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser 14-3-3 sigma/SFN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-14-3-3 sigma/SFN Antikörper

Bindungsspezifität: AA 1-248

Verwendungszweck: Anti-14-3-3 sigma/SFN Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-14-3-3 sigma/SFN Antibody (ABIN5693156). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-14-3-3 sigma/SFN Antikörper (ABIN5693156)

Yang, Cheung, Peng, He, Wu, Xie, Jiang, Huang, Chen, Lin, Kung: "Makorin-2 is a neurogenesis inhibitor downstream of phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8486-95, (2008) (PubMed).

Details zu 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Andere Bezeichnung: SFN

Hintergrund:

Synonyms: 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1

Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Background: Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Molekulargewicht: 28 kDa

Gen-ID: 2810

UniProt: P31947

Pathways: p53 Signalweg, Myometrial Relaxation and Contraction