ICOS Antikörper (AA 21-140)

Produktnummer ABIN5693002

Der Kaninchen Polyklonal Anti-ICOS-Antikörper wurde für IHC, FACS, ELISA und ICC validiert. Er ist geeignet, ICOS in Proben von Human zu detektieren.

Kurzübersicht für ICOS Antikörper (AA 21-140) (ABIN5693002)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ICOS Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-ICOS Antikörper

Bindungsspezifität: AA 21-140

Verwendungszweck: Anti-ICOS Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ICOS Antibody Picoband® (ABIN5693002). Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Immunogen: E. coli-derived human ICOS recombinant protein (Position: E21-K140).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Hutloff A, Dittrich AM, Beier KC, Eljaschewitsch B, Kraft R, Anagnostopoulos I, Kroczek RA (Jan 1999). "ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28". Nature. 397(6716): 263-6. 2. Rudd CE, Schneider H (Jul 2003). "Unifying concepts in CD28, ICOS and CTLA4 co-receptor signalling". Nature Reviews. Immunology. 3 (7): 544-56.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Andere Bezeichnung: ICOS

Hintergrund:

Synonyms: Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278, ICOS, AILIM

Tissue Specificity: Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

Background: Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Molekulargewicht: 20 kDa

Gen-ID: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints