APEX1 Antikörper (AA 2-318)
-
- Target Alle APEX1 Antikörper anzeigen
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
-
Bindungsspezifität
- AA 2-318
-
Reaktivität
- Human
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser APEX1 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Marke
- Picoband™
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
- Mouse IgG monoclonal antibody for APE1 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.
- Immunogen
- E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.
- Klon
- 5C11
- Isotyp
- IgG2b
- Top Product
- Discover our top product APEX1 Primärantikörper
-
-
- Applikationshinweise
-
Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry, 1-3 μg/1x106 cells - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
-
- Target
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- Andere Bezeichnung
- APEX1 (APEX1 Produkte)
- Synonyme
- apex1 antikoerper, MGC76280 antikoerper, MGC79645 antikoerper, MGC78928 antikoerper, APE antikoerper, APE1 antikoerper, APEN antikoerper, APEX antikoerper, APX antikoerper, HAP1 antikoerper, REF1 antikoerper, Apex antikoerper, REF-1 antikoerper, BAP1 antikoerper, apex1a antikoerper, hm:zehn1532 antikoerper, wu:fc13d11 antikoerper, zgc:66204 antikoerper, Ref-1 antikoerper, apurinic/apyrimidinic endodeoxyribonuclease 1 antikoerper, APEX nuclease (multifunctional DNA repair enzyme) 1 antikoerper, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antikoerper, DNA-(apurinic or apyrimidinic site) lyase antikoerper, apurinic/apyrimidinic endonuclease 1 antikoerper, APEX1 antikoerper, apex1 antikoerper, apex1.L antikoerper, CNG03640 antikoerper, CNH01280 antikoerper, nth antikoerper, Spirs_4119 antikoerper, Palpr_0103 antikoerper, Apex1 antikoerper
- Hintergrund
-
Synonyms: DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1
Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
- UniProt
- P27695
- Pathways
- DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
-