GFP Antikörper

Produktnummer ABIN5691123

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch GFP in WB, ELISA, IF und ICC. Er zeigt eine Reaktivität gegenüber Aequorea victoria.

Kurzübersicht für GFP Antikörper (ABIN5691123)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-GFP Antikörper

Spezifität: The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.

Kreuzreaktivität (Details): Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology, and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols. The antibody is also suitable for immunocytochemical detection of proteins labeled with the different variants of GFP.

Aufreinigung: Immunoaffinity purified

Immunogen: The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The antibody is suitable for the detection of GFP and its variants by Western blotting, ELISA and Immunocytochemistry. Optimal antibody dilutions for the different applications should be determined by titration. Recommended dilution for ELISA: 1:5,000 to 1:25,000. For immunoblotting a dilution of 1:2,000 to 1:10,000 is recommended. For immunocytochemistry the antiserum can be diluted 1:100 to 1:250.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Each vial contains 25 μg 1 mg/mL immunoaffinity purified polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C, or in small aliquots at -20°C for storage during longer periods

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: Green Fluorescent Protein

Hintergrund: Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology, and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols. The antibody is also suitable for immunocytochemical detection of proteins labeled with the different variants of GFP.