Perforin 1 Antikörper (AA 98-534)

Produktnummer ABIN5690663

Dieses Anti-Perforin 1-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Perforin 1 in WB, IHC, IP und ICC. Geeignet für Maus.

Kurzübersicht für Perforin 1 Antikörper (AA 98-534) (ABIN5690663)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Perforin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: CB5-4

Produktdetails anti-Perforin 1 Antikörper

Bindungsspezifität: AA 98-534

Aufreinigung: Perforin (monoclonal CB5.4) antibody was purified to ≥,95% in SDS-PAGE.

Immunogen: Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1 % BSA, slides were incubated with 5 μ,g/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Perforin antibody can be stored at 4°C, stable for one year.

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin

Gen-ID: 18646

UniProt: P10820

Pathways: Apoptose, Caspase Kaskade in der Apoptose