CTGF Antikörper

Produktnummer ABIN5689933

Der Kaninchen Polyklonal anti-CTGF Antikörper wird verwendet zum Nachweis von CTGF in Proben von Human. Er wurde validiert für WB, ELISA und Neut.

Kurzübersicht für CTGF Antikörper (ABIN5689933)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CTGF Antikörper

Aufreinigung: CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 μ,g/mL), a concentration of 6.0 - 9.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hCTGF by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.
Sandwich:
To detect hCTGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required.

Western Blot:
To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: CTGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Andere Bezeichnung: CTGF

Hintergrund: CTGF (connective tissue growth factor) is a 38  kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.

Gen-ID: 1490

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding