EIF2A Antikörper (pSer51)

Produktnummer ABIN5689682

Der Kaninchen Polyklonal anti-EIF2A Antikörper (ABIN5689682) detektiert spezifisch EIF2A in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für EIF2A Antikörper (pSer51) (ABIN5689682)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-EIF2A Antikörper

Bindungsspezifität: pSer51

Spezifität: This antibody detects endogenous level of eIF2α, only when phosphorylated at serine 51.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: eIF2a (Phospho-Ser51) antibody was raised against a peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2α,.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Details zu EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Andere Bezeichnung: eIF2a

Hintergrund: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Molekulargewicht: 38 kDa

Gen-ID: 1965

NCBI Accession: NP_004085

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly