KIT Ligand Antikörper

Produktnummer ABIN5689173

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KIT Ligand in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für KIT Ligand Antikörper (ABIN5689173)

Target: KIT Ligand (KITLG)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-KIT Ligand Antikörper

Aufreinigung: Anti-mSCF specific antibody was purified by affinity chromatography employing immobilized mSCF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μ,g/mL of this antibody is required.

ELISA:

To detect mSCF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF.

Sandwich:

To detect mSCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.

Western Blot:

To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: SCF

Gen-ID: 17311

UniProt: P20826

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung