KIT Ligand Antikörper

Produktnummer ABIN5689173

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch KIT Ligand in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für KIT Ligand Antikörper (ABIN5689173)

Target: KIT Ligand (KITLG)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-KIT Ligand Antikörper

Aufreinigung: Anti-mSCF specific antibody was purified by affinity chromatography employing immobilized mSCF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μ,g/mL of this antibody is required.

ELISA:

To detect mSCF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF.

Sandwich:

To detect mSCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.

Western Blot:

To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: SCF

Gen-ID: 17311

UniProt: P20826

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung