Glutamate Receptor 1 Antikörper (pSer845)

Produktnummer ABIN5688909

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Glutamate Receptor 1 in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für Glutamate Receptor 1 Antikörper (pSer845) (ABIN5688909)

Target: Glutamate Receptor 1 (GLUR1)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glutamate Receptor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Glutamate Receptor 1 Antikörper

Bindungsspezifität: pSer845

Spezifität: GluR1 antibody is specific for GluR1 phosphorylated at Ser845.

Aufreinigung: Affinity Purified

Immunogen: GluR1 (Ser845) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser845 of rat GluR1.

Anwendungsinformationen

Applikationshinweise: Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Details zu Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Andere Bezeichnung: GluR1

Hintergrund: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of 4 distinct subunits (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser845. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.

Molekulargewicht: 100 kDa

Gen-ID: 50592

UniProt: P19490

Pathways: PI3K-Akt Signalweg