Tryptophan Hydroxylase 1 Antikörper (pSer19)

Produktnummer ABIN5688890

Dieses Anti-Tryptophan Hydroxylase 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Tryptophan Hydroxylase 1 in WB. Geeignet für Maus, Ratte, Rind (Kuh) und Xenopus laevis.

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (pSer19) (ABIN5688890)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Maus, Ratte, Rind (Kuh), Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Bindungsspezifität: pSer19

Aufreinigung: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat tryptophan hydroxylase 2 (TPH2).

Anwendungsinformationen

Applikationshinweise: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, mouse and zebra fish based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Tryptophan Hydroxylase antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: Tryptophan Hydroxylase

Hintergrund: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et

Molekulargewicht: 55 kDa

Gen-ID: 317675

UniProt: Q8CGU9

Pathways: Feeding Behaviour