Fibrillarin Antikörper

Produktnummer ABIN5688751

Der Maus Monoklonal Anti-Fibrillarin-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Fibrillarin in Proben von Human, Ratte und Drosophila melanogaster zu detektieren.

Kurzübersicht für Fibrillarin Antikörper (ABIN5688751)

Target: Fibrillarin (FBL)

Reaktivität: Human, Ratte, Drosophila melanogaster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 38F3

Produktdetails anti-Fibrillarin Antikörper

Spezifität: Specific for the ~34  kDa Fibrillarin /Nop1p protein.

Aufreinigung: Total IgG fraction

Immunogen: Yeast nuclear preparations.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: -20 °C

Informationen zur Lagerung: Fibrillarin antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: Fibrillarin

Hintergrund: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Molekulargewicht: 34 kDa

Gen-ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process