NGFB Antikörper

Produktnummer ABIN5688734

Dieses Anti-NGFB-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NGFB in WB, ELISA und Neut. Geeignet für Human.

Kurzübersicht für NGFB Antikörper (ABIN5688734)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Klon: M85-2B

Produktdetails anti-NGFB Antikörper

Aufreinigung: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 200 pg/mL of recombinant human β,-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hβ,-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ,-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Details zu NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Andere Bezeichnung: bNGF

Gen-ID: 4803

UniProt: P01138

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size