GZMB Antikörper (AA 7-227)

Produktnummer ABIN5688512

Dieses Anti-GZMB-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GZMB in WB, IHC, FACS, IP und ICC. Geeignet für Human.

Kurzübersicht für GZMB Antikörper (AA 7-227) (ABIN5688512)

Target: GZMB (Granzyme B (GZMB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: B18-1

Produktdetails anti-GZMB Antikörper

Bindungsspezifität: AA 7-227

Aufreinigung: Granzyme B (monoclonal B18.1) antibody was purified to ≥,95% in SDS-PAGE.

Immunogen: Granzyme B (monoclonal B18.1) antibody was raised against recombinant human granzyme B, amino acids 7-227.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % Fetal calf serum, 0.02 % azide) containing 1 μ,g of FITC- labeled B18.1 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1 % BSA, slides were incubated for 1 hr at room temperature (RT) with 20 μ,g/mL of B18.4 antibody in PBS, 0.1 % BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Granzyme B antibody can be stored at 4°C, stable for one year.

Details zu GZMB

Target: GZMB (Granzyme B (GZMB))

Andere Bezeichnung: Granzyme B

Gen-ID: 3002

UniProt: P10144

Pathways: Apoptose, Caspase Kaskade in der Apoptose