KIT Ligand Antikörper (Biotin)

Produktnummer ABIN5687609

Dieses Anti-KIT Ligand-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von KIT Ligand in WB und ELISA. Geeignet für Human.

Kurzübersicht für KIT Ligand Antikörper (Biotin) (ABIN5687609)

Target: KIT Ligand (KITLG)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-KIT Ligand Antikörper (Biotin)

Aufreinigung: Anti-hSCF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSCF (human Stem Cell Factor).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect hSCF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.

Sandwich:

To detect hSCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human SCF (XP-5265) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.

Western Blot:

To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: SCF

Hintergrund: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

Gen-ID: 4254

UniProt: P21583

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung