CD40 Ligand Antikörper (Biotin)

Produktnummer ABIN5687607

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CD40 Ligand in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD40 Ligand Antikörper (Biotin) (ABIN5687607)

Target: CD40 Ligand (CD40LG)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CD40 Ligand Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CD40 Ligand Antikörper (Biotin)

Aufreinigung: Anti-hsCD40L specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L (Human sCD40L).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect hsCD40L by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsCD40L.

Sandwich:

To detect hsCD40L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L.

Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CD40 Ligand

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: sCD40 Ligand

Gen-ID: 959

UniProt: P29965

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints