C3 Antikörper (HRP)

Produktnummer ABIN568554

Dieses Anti-C3-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von C3 in WB, IP, EIA, IF, ID und DB. Geeignet für Meerschweinchen.

Kurzübersicht für C3 Antikörper (HRP) (ABIN568554)

Target: C3 (Complement Component 3 (C3))

Reaktivität: Meerschweinchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser C3 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID), Dot Blot (DB)

Produktdetails anti-C3 Antikörper (HRP)

Spezifität: In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of guinea pig serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of guinea pig plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Produktmerkmale: Molar Ratio: Peroxidase/IgG ~1.2

Aufreinigung: Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography

Immunogen: C3c is isolated and purified from pooled normal guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1 mL of sterile distilled water.

Konzentration: 10,0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

Details zu C3

Target: C3 (Complement Component 3 (C3))

Andere Bezeichnung: Complement C3

Hintergrund: C3 is the most abundant complement protein in guinea pig serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.Synonyms: CPAMD1, Complement component 3

Gen-ID: 24232

NCBI Accession: NP_058690

UniProt: P01026

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling